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题名: Label-Free MicroRNA Detection Based on Fluorescence Quenching of Gold Nanoparticles with a Competitive Hybridization
作者: Wang, W; Kong, T(孔涛); Zhang, D(张栋); Zhang, J; Cheng, GS(程国胜)
通讯作者: Cheng, GS (程国胜)
刊名: ANALYTICAL CHEMISTRY
发表日期: 2015
DOI: 10.1021/acs.analchem.5b01930
卷: 87, 期:21, 页:8
收录类别: SCI
部门归属: 纳米生物医学与安全研究部
英文摘要: MicroRNAs (miRNAs), critical biomarkers of acute and chronic diseases, play key regulatory roles in many biological processes. As a result, there is great demand for robust assay platforms to enable an accurate and efficient detection of low-level miRNAs in complex biological samples. In this work, a label-free and Au nanopartides (NPs) quenching-based competition assay system was developed. In the designed system, Au NPs with diameter sizes of 10 and 20 nm displayed fluorescence quenching efficiencies of 84% and 82% for Cy3 dye on slide surface, whereas the quenching efficiency of commercial BHQ2 quencher was roughly 50%. Assay conditions were optimized for miRNA-205 detection. A limit of detection of 3.8 pM and a detection range covering from 3.8 pM to 10 nM were achieved. Furthermore, the proposed system was capable of specifically discriminating miRNAs with slight variations in their nucleotide sequence and was also qualified for assessing miRNA levels in human serum. Our strategy has the potential to provide new perspectives in profiling the pattern of miRNA expression and biomedical utilizations.
语种: 英语
JCR小类分区: 二区
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.sinano.ac.cn/handle/332007/3277
Appears in Collections:纳米生物医学与安全研究部_程国胜团队_期刊论文

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Recommended Citation:
Wang, W,Kong, T,Zhang, D,et al. Label-Free MicroRNA Detection Based on Fluorescence Quenching of Gold Nanoparticles with a Competitive Hybridization[J]. ANALYTICAL CHEMISTRY,2015,87(21):8.
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