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题名: A Cellular Compatible Chitosan Nanoparticle Surface for Isolation and In Situ Culture of Rare Number CTCs
作者: Sun, N(孙娜); Wang, J; Ji, LY(纪利亚); Hong, SN(洪珊妮); Dong, JJ(董京金); Guo, YH(郭亚辉); Zhang, KC(张坤驰); Pei, RJ(裴仁军)
通讯作者: Pei, RJ (裴仁军)
关键词: biomaterials ; chitosan ; circulating tumor cells ; in situ culture ; nanoparticles
刊名: SMALL
发表日期: 2015
DOI: 10.1002/smll.201501718
卷: 11, 期:40, 页:8
收录类别: SCI
部门归属: 纳米生物医学与安全研究部
英文摘要: Circulating tumor cell (CTC) isolation has attracted a great deal of research interest in recent years. However, there are still some challenges, including purity as well as viability of the captured CTCs, resulting from nanoscale structures and inorganic nanomaterials. Here, a chitosan nanoparticle surface is first fabricated by electrospray to provide a cellular compatible interface. The soft substrate, further modified by polyethylene glycol (PEG) as an antifouling molecule and DNA aptamer as a specific capture molecule, has a hydrophilic nature and is capable of specific capture of viable rare CTCs from artificial white blood cell (WBC) samples. Furthermore, a subsequent in situ culture strategy based on the developed cellular compatible soft interface is introduced for further purification and proliferation of the captured rare number target cells. The WBCs are weeded out after 2 d, and after a 7 d proliferation nearly 200 MCF-7 cells are obtained from 7 target cells with more than 90% purity. This work provides a promising strategy for viable isolation and purification of rare CTCs and it has great potential for achieving clinical validity.
语种: 英语
JCR小类分区: 一区
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.sinano.ac.cn/handle/332007/3288
Appears in Collections:纳米生物医学与安全研究部_裴仁军团队_期刊论文

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Recommended Citation:
Sun, N,Wang, J,Ji, LY,et al. A Cellular Compatible Chitosan Nanoparticle Surface for Isolation and In Situ Culture of Rare Number CTCs[J]. SMALL,2015,11(40):8.
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