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题名: An aptameric graphene nanosensor for label-free detection of small-molecule biomarkers
作者: Wang, C; Kim, J; Zhu, YB; Yang, JY; Lee, GH; Lee, S; Yu, J; Pei, RJ(裴仁军); Liu, GH; Nuckolls, C; Hone, J; Lin, Q
通讯作者: Lin, Q
关键词: Aptamer ; Competitive assay ; Dehydroepiandrosterone sulfate (DHEA-S) ; Graphene ; Nanobiosensor ; Small molecule
刊名: BIOSENSORS & BIOELECTRONICS
发表日期: 2015
DOI: 10.1016/j.bios.2015.04.025
卷: 71, 页:8
收录类别: SCI
部门归属: 纳米生物医学与安全研究部
英文摘要: This paper presents an aptameric graphene nanosensor for detection of small-molecule biomarkers. To address difficulties in direct detection of small molecules associated with their low molecular weight and electrical charge, we incorporate an aptamer-based competitive affinity assay in a graphene field effect transistor (FET), and demonstrate the utility of the nanosensor with dehydroepiandrosterone sulfate (DHEA-S), a small-molecule steroid hormone, as the target analyte. In the competitive affinity assay, DHEA-S specifically binds to aptamer molecules pre-hybridized to their complementary DNA anchor molecules immobilized on the graphene surface. This results in the competitive release of the strongly charged aptamer from the DNA anchor and hence a change in electrical properties of the graphene, which can be measured to achieve the detection of DHEA-S. We present experimental data on the labelfree, specific and quantitative detection of DHEA-S at clinically appropriate concentrations with an estimated detection limit of 44.7 nM, and analyze the trend observed in the experiments using molecular binding kinetics theory. These results demonstrate the potential of our nanosensor in the detection of DHEA-S and other small molecules in biomedical applications. (C) 2015 Elsevier B.V. All rights reserved.
语种: 英语
JCR小类分区: 一区
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内容类型: 期刊论文
URI标识: http://ir.sinano.ac.cn/handle/332007/3312
Appears in Collections:纳米生物医学与安全研究部_裴仁军团队_期刊论文

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Recommended Citation:
Wang, C,Kim, J,Zhu, YB,et al. An aptameric graphene nanosensor for label-free detection of small-molecule biomarkers[J]. BIOSENSORS & BIOELECTRONICS,2015,71:8.
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