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题名: A collagen-binding EGFR single-chain Fv antibody fragment for the targeted cancer therapy
作者: Liang, H(梁辉); Li, XR(李晓然); Chen, B; Wang, B; Zhao, YN; Zhuang, Y(庄燕); Shen, H(沈贺); Zhang, ZJ(张智军); Dai, JW(戴建武)
通讯作者: Dai, JW (戴建武)
关键词: Collagen ; Collagen-binding domain ; CBD-scFv ; Controlled release ; A431 xenograft
刊名: JOURNAL OF CONTROLLED RELEASE
发表日期: 2015
DOI: 10.1016/j.jconrel.2015.04.029
卷: 209, 页:9
收录类别: SCI
部门归属: 纳米生物医学与安全研究部
英文摘要: Collagen, a primary component of the extracellular matrix (ECM), is highly expressed in a variety of cancers and influences the tumor microenvironment by increasing the recruitment of macrophages and endothelial cells. Therefore, collagen is a highly promising target for cancer therapy. The collagen-binding domain (CBD) can dynamically bind to collagen and achieve the sustained release of CBD-fused protein in the collagen network. Here, we developed a collagen-binding epidermal growth factor receptor (EGFR) antibody fragment for targeting the collagen-rich ECM in tumors. The single chain fragment variable (scFv) of cetuximab was fused to CBD (CBD-scFv) and expressed in Pichia pastoris. CBD-scFv preserved the antigen binding domain and anti-tumor activity of cetuximab in vitro. Moreover, CBD-scFv displayed a collagen binding ability due to the function of CBD. In vivo experiments revealed that CBD-scFv bound to collagen and achieved sustained release in tumors. Furthermore, CBD-scFv significantly suppressed the growth of tumors in A431 xenografts. Therefore, CBD-scFv had a potential therapeutic value for the collagen-rich carcinomas. The specific target and sustained release of CBD-scFv in tumors could be a new approach for targeted drug delivery in cancer therapy. (C) 2015 Elsevier B.V. All rights reserved.
语种: 英语
JCR小类分区: 一区
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内容类型: 期刊论文
URI标识: http://ir.sinano.ac.cn/handle/332007/3340
Appears in Collections:纳米生物医学与安全研究部_戴建武团队_期刊论文

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Recommended Citation:
Liang, H,Li, XR,Chen, B,et al. A collagen-binding EGFR single-chain Fv antibody fragment for the targeted cancer therapy[J]. JOURNAL OF CONTROLLED RELEASE,2015,209:9.
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