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题名: One-step synthesis of biofunctional carbon quantu`m dots for bacterial labeling
作者: Weng, CI; Chang, HT; Lin, CH(林家骅); Shen, YW; Unnikrishnan, B; Li, YJ; Huang, CC
通讯作者: Huang, CC
关键词: Carbon quantum dots ; Mannose ; Bacterial labeling ; Lectin ; E. coli
刊名: BIOSENSORS & BIOELECTRONICS
发表日期: 2015
DOI: 10.1016/j.bios.2014.12.028
卷: 68, 页:6
收录类别: SCI
部门归属: 纳米生物医学与安全研究部
英文摘要: In this study, we used a simple one-step dry heating method to synthesize mannose-modified fluorescent carbon quantum dots (Man-CQDs) from solid ammonium citrate and mannose, and successfully applied for labeling Escherichia coli. The highly soluble Man-CQDs had an average particle diameter of 3.1 +/- 1.2 nm and exhibited a quantum yield of 9.8% at excitation and emission wavelengths of 365 and 450 nm, respectively. The fluorescent Man-CQDs could selectively bind to the FimH lectin unit in the flagella of the wild-type 1 E. coli K12 strain. We optimized the labeling efficiency of the Man-CQDs by controlling the ratio of ammonium citrate to mannose during their synthesis. The specific binding of the mannose units to E. coli allowed quantitative detection of the bacteria at levels down to 450 colony forming units mL(-1) in lab samples, and facilitate the application of the Man-CQDs for bacterial analyses of real samples (tap water, apple juice, human urine). The synthesis of our Man-CQDs, their labeling, and their use in the detection of bacteria were all simple, inexpensive and efficient processes. (C) 2014 Elsevier B.V. All rights reserved.
语种: 英语
JCR小类分区: 一区
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内容类型: 期刊论文
URI标识: http://ir.sinano.ac.cn/handle/332007/3354
Appears in Collections:纳米生物医学与安全研究部_离职团队_期刊论文

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Recommended Citation:
Weng, CI,Chang, HT,Lin, CH,et al. One-step synthesis of biofunctional carbon quantu`m dots for bacterial labeling[J]. BIOSENSORS & BIOELECTRONICS,2015,68:6.
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