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题名: An ultralow background substrate for protein microarray technology
作者: Feng, H; Zhang, QY; Ma, HW(马宏伟); Zheng, B
通讯作者: Zheng, B
刊名: ANALYST
发表日期: 2015
DOI: 10.1039/c5an00852b
卷: 140, 期:16, 页:7
收录类别: SCI
部门归属: 纳米生物医学与安全研究部
英文摘要: We herein report an ultralow background substrate for protein microarrays. Conventional protein microarray substrates often suffer from non-specific protein adsorption and inhomogeneous spot morphology. Consequently, surface treatment and a suitable printing solution are required to improve the microarray performance. In the current work, we improved the situation by developing a new microarray substrate based on a fluorinated ethylene propylene (FEP) membrane. A polydopamine microspot array was fabricated on the FEP membrane, with proteins conjugated to the FEP surface through polydopamine. Uniform microspots were obtained on FEP without the application of a special printing solution. The modified FEP membrane demonstrated ultralow background signal and was applied in protein and peptide microarray analysis.
语种: 英语
JCR小类分区: 二区
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.sinano.ac.cn/handle/332007/3467
Appears in Collections:纳米生物医学与安全研究部_马宏伟团队_期刊论文

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Recommended Citation:
Feng, H,Zhang, QY,Ma, HW,et al. An ultralow background substrate for protein microarray technology[J]. ANALYST,2015,140(16):7.
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