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题名: Identification of VEGFR2-Binding Peptides Using High Throughput Bacterial Display Methods and Functional Assessment
作者: Pu, KF(濮科锋); Yuan, LH(原丽华); Chen, LS(陈丽莎); Wang, AX(王安欣); Zhou, X(周旋); Zhang, HL(张海禄); Zhu, YM(朱毅敏)
通讯作者: Zhu, YM (朱毅敏)
关键词: Angiogenesis ; bacterial surface display ; cancer diagnosis ; cancer therapy ; peptide ; vascular endothelial growth factor receptor 2 (VEGFR2)
刊名: CURRENT CANCER DRUG TARGETS
发表日期: 2015
卷: 15, 期:2, 页:13
收录类别: SCI
部门归属: 纳米生物医学与安全研究部
英文摘要: The signal transduction pathway initiated by vascular endothelial growth factor-vascular endothelial growth factor receptor 2 (VEGF-VEGFR2) plays an important role in the angiogenesis of tumors. The effective antagonists of VEGFR2 would behave as potent drugs for the treatment of malignant cancers. In our study, specific binding peptides with high affinity to VEGFR2 were obtained through bacterial display technology. Conserved motif (FF/YEXWGVK) among those peptide sequences was discovered. One of the selected peptides, VRBP1 (YDGNSFYEMWGVKPASES) was identified by screening the biased bacterial peptide library and its physiochemical feature was further characterized. The results of surface plasmon resonance (SPR) assay indicated that the dissociation constant (K-D) value of VRBP1 was 228.3 nM and this peptide competed with VEGF binding to VEGFR2. Particles conjugated with VRBP1 could recognize the human umbilical vein endothelial cells (HUVEC) which express VEGFR2 on the surface. Further therapeutic effect of VRBP1 was examined by in vivo experiments. VRBP1 could result in a significant decrease in tumor size of H460 xenografts. The results from the immunohistochemical assay showed that CD31 positive signals in VRBP1-treated group were fewer than those in the control ones. These data highlighted the potential of VEGFR2-binding peptides as effective molecules for cancer diagnosis and therapy.
语种: 英语
JCR小类分区: 二区
内容类型: 期刊论文
URI标识: http://ir.sinano.ac.cn/handle/332007/3482
Appears in Collections:纳米生物医学与安全研究部_朱毅敏团队_期刊论文

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Recommended Citation:
Pu, KF,Yuan, LH,Chen, LS,et al. Identification of VEGFR2-Binding Peptides Using High Throughput Bacterial Display Methods and Functional Assessment[J]. CURRENT CANCER DRUG TARGETS,2015,15(2):13.
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