中国科学院苏州纳米技术与纳米仿生研究所机构知识库
Advanced  
SINANO OpenIR  > 纳米生物医学与安全研究部  > 裴仁军团队  > 期刊论文
题名: Selection and analysis of DNA aptamers to berberine to develop a label-free light-up fluorescent probe
作者: Wang, JE(王金娥); Zhang, YJ; Wang, HY(王红沿); Chen, Y(陈阳); Xu, LJ(许丽君); Gao, T; Pei, RJ(裴仁军)
通讯作者: Pei, RJ(裴仁军)
刊名: NEW JOURNAL OF CHEMISTRY
发表日期: 2016
DOI: 10.1039/c6nj02290a
卷: 40, 期:11
收录类别: SCI ; EI
文章类型: 期刊论文
部门归属: 纳米生物医学与安全研究部
英文摘要: Aptamers are short single-stranded DNA or RNA, which can be selected from a random combinatorial library by the systematic evolution of ligands by exponential enrichment (SELEX) in vitro. In order to obtain novel selective light-up ligands for berberine, we selected DNA aptamers for berberine by a modified affinity chromatography-based SELEX. The ssDNA library was coupled on streptavidin-coated agarose beads through the hybridization with a biotin labeled complementary oligonucleotide capture strand, and the aptamer sequences were released from agarose beads through berberine binding. This method to isolate the aptamers was simple, straightforward and effective, and three aptamers with low micromolar dissociation constants (K(d)s) were achieved after only 6 rounds of selection. The best of them was further optimized, and the shortened aptamers showed similar K(d)s to the full length sequences. The light-up phenomenon of the berberine-aptamer pair was also investigated and the final minimized 21-mer aptamer which showed higher fluorescence enhancement than G-quadruplex structures could be used as a novel light-up fluorescent probe for various biosensing applications.
关键词[WOS]: G-QUADRUPLEX DNA ; IN-VITRO ; MALACHITE GREEN ; DOWN-REGULATION ; RNA ; DERIVATIVES ; LIGANDS ; BINDING ; DYES ; INHIBITION
语种: 英语
JCR小类分区: 三区
WOS记录号: WOS:000387621500091
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.sinano.ac.cn/handle/332007/4681
Appears in Collections:纳米生物医学与安全研究部_裴仁军团队_期刊论文

Files in This Item:
File Name/ File Size Content Type Version Access License
Wang-2016-Selection and analysis of DNA aptame.pdf(2094KB)期刊论文作者接受稿限制开放View 联系获取全文

Recommended Citation:
Wang, JE,Zhang, YJ,Wang, HY,et al. Selection and analysis of DNA aptamers to berberine to develop a label-free light-up fluorescent probe[J]. NEW JOURNAL OF CHEMISTRY,2016,40(11).
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Wang, JE(王金娥)]'s Articles
[Zhang, YJ]'s Articles
[Wang, HY(王红沿)]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Wang, JE(王金娥)]‘s Articles
[Zhang, YJ]‘s Articles
[Wang, HY(王红沿)]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: Wang-2016-Selection and analysis of DNA aptame.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!