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题名: An electrochemical aptasensor electrocatalyst for detection of thrombin
作者: Tian, R; Chen, XJ; Li, QW(李清文); Yao, C
通讯作者: Tian, R ; Yao, C
刊名: ANALYTICAL BIOCHEMISTRY
发表日期: 2016
DOI: 10.1016/j.ab.2016.01.021
卷: 500
收录类别: SCI
文章类型: 期刊论文
部门归属: 先进材料研究部
英文摘要: This work reports a novel signal amplification strategy based on three-dimensional ordered macroporous C-60-poly(3,4-ethylenedioxythiophene)-1-butyl-3-methylimidazolium hexafluorophosphate (3DOM C-60-PEDOT-[BMIm][BF6]) for ultrasensitive detection of thrombin by cascade catalysis of Au-PEDOT@SiO2 microspheres and alcohol dehydrogenase (ADH). Au-PEDOT@SiO2 microspheres were constructed not only as nanocarriers to anchor the large amounts of secondary thrombin aptamers but also as nano catalysts to catalyze the oxidation of ethanol efficiently. Significantly, the electrochemical signal was greatly enhanced based on cascade catalysis: First, ADH catalyzed the oxidation of ethanol to acetaldehyde with the concomitant generation of NADH in the presence of beta-nicotinamide adenine dinucleotide hydrate (NADI(+)). Then, gold nanoparticles (AuNPs) as nanocatalysts could effectively catalyze NADH to produce NAD(+) with the help of PEDOT as redox probe. Under the optimal conditions, the proposed aptasensor exhibits a linear range of 2 x 10(-13) to 2 x 10(-8) M with a low detection limit of 2 x 10(-14) M for thrombin detection and shows high sensitivity and good specificity. (C) 2016 Elsevier Inc. All rights reserved.
关键词[WOS]: HIGHLY SENSITIVE DETECTION ; GOLD NANOPARTICLES ; CARBON NANOTUBE ; AMPLIFICATION APTASENSOR ; DUAL-AMPLIFICATION ; SOLAR-CELLS ; ELECTRODE ; MICROSPHERES ; POLYANILINE ; QUADRUPLEX
语种: 英语
JCR小类分区: 三区
WOS记录号: WOS:000373968700015
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.sinano.ac.cn/handle/332007/4699
Appears in Collections:先进材料研究部_李清文团队_期刊论文

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Recommended Citation:
Tian, R,Chen, XJ,Li, QW,et al. An electrochemical aptasensor electrocatalyst for detection of thrombin[J]. ANALYTICAL BIOCHEMISTRY,2016,500.
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