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题名: Enhanced Migration of Neural Stem Cells by Microglia Grown on a Three-Dimensional Graphene Scaffold
作者: Jiang, ZY(姜自云); Song, Q(宋琴); Tang, ML(唐明亮); Yang, LY(杨令延); Cheng, YL(程一琳); Zhang, M; Xu, DS; Cheng, GS(程国胜)
通讯作者: Cheng, GS(程国胜)
刊名: ACS APPLIED MATERIALS & INTERFACES
发表日期: 2016
DOI: 10.1021/acsami.6b06780
卷: 8, 期:38
收录类别: SCI ; EI
文章类型: 期刊论文
部门归属: 纳米生物医学与安全研究部
英文摘要: One of the key challenges in engineering neural tissues for cell-based therapies is to develop a biocompatible scaffold material to direct neural stem cell (NSC) behaviors. One great advantage for a scaffold would be to induce NSC migration toward pathological sites during regeneration and repair. In particular, the inflammatory responses in the pathological zone, which are mainly mediated by microglia in the central nervous system, affect the repair capacity of NSCs through NSC migration. Recently, graphene was used as a neural interface and scaffold material, but few studies have addressed the relationship between microglia. and NSCs in a graphene culture system. In this study, we used a combination of immunofluorescence, Western blotting, :enzyme-linked immunosorbent assays, and scanning electron microscopy to investigate how conditioned medium (CM) produced from microglia grown on two-dimensional graphene (2D-G) films or three-dimensional graphene (3D-G) foams govern NSC migration. The results revealed that the CM produced by microglia grown in 3D-G cultures could promote neurosphere formation, facilitate NSC migration from the neurospheres, and increase single cell polarization by activating the stromal cell-derived factor 1 alpha (SDF-1 alpha)/CXC chemokine-receptor 4 (CXCR4) signaling pathway and enhancing cell adhesion on the substrate. By contrast, the 2D-G CM failed to achieve these results. Our study suggests the great potential of 3D-G as a neural scaffold for NSC-based therapy in tissue engineering and regenerative medicine.
关键词[WOS]: RAT SUBVENTRICULAR ZONE ; OSTEOGENIC DIFFERENTIATION ; STRIPE ASSAY ; BRAIN-INJURY ; OXIDE ; PHOSPHORYLATION ; PROLIFERATION ; GLIOBLASTOMA ; INFLAMMATION ; RECRUITMENT
语种: 英语
JCR小类分区: 一区
WOS记录号: WOS:000384518500015
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.sinano.ac.cn/handle/332007/4835
Appears in Collections:纳米生物医学与安全研究部_程国胜团队_期刊论文

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Recommended Citation:
Jiang, ZY,Song, Q,Tang, ML,et al. Enhanced Migration of Neural Stem Cells by Microglia Grown on a Three-Dimensional Graphene Scaffold[J]. ACS APPLIED MATERIALS & INTERFACES,2016,8(38).
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