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题名: Building a chimera of aptamer-antisense oligonucleotide for silencing galectin-1 gene
作者: Hong, Shanni(洪珊妮); Sun, Na(孙娜); Liu, Min(刘敏); Wang, Jine(王金娥); Pei, Renjun(裴仁军)
通讯作者: Liu, M(刘敏) ; Pei, RJ(裴仁军) 
刊名: RSC Advances
发表日期: 2016
DOI: 10.1039/c6ra21250f
卷: 6, 期:113
收录类别: SCI ; EI
文章类型: 期刊论文
部门归属: 纳米生物医学与安全研究部
英文摘要: Galectin-1 is closely related with immune systems, and its overexpression may cause tumor metastasis. Owing to the better stability of antisense oligonucleotides than siRNA, we constructed a chimera of aptamer-antisense oligonucleotide (Apt-AS) for silencing the galectin-1 gene specifically. For studying the endocytosis pathways of Apt-AS by aptamer targeted delivery, we marked the 3' terminus of Apt-AS with Alexa Flour 488 (Apt-AS488) so as to trace the pathways through the confocal microscope. Furthermore, Apt-AS was used to silence the expression of galectin-1. The results showed Apt-AS entered cells by a caveolae mediated endocytosis pathway, and the aptamer-antisense chimeras did not impair the antisense gene silencing efficiency. Moreover Apt-AS could improve the cellular uptake and selectively entered cells.
关键词[WOS]: TARGETED CANCER-THERAPY ; INTRACELLULAR DELIVERY ; DNA NANOSTRUCTURES ; IN-VIVO ; SIRNA ; CELLS ; EFFICIENT ; VEHICLES
语种: 英语
JCR小类分区: 二区
WOS记录号: WOS:000389708700092
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.sinano.ac.cn/handle/332007/4861
Appears in Collections:纳米生物医学与安全研究部_裴仁军团队_期刊论文

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Recommended Citation:
Hong, Shanni,Sun, Na,Liu, Min,et al. Building a chimera of aptamer-antisense oligonucleotide for silencing galectin-1 gene[J]. RSC Advances,2016,6(113).
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