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题名: Improved osteogenesis and upregulated immunogenicity in human placenta-derived mesenchymal stem cells primed with osteogenic induction medium
作者: Fu, XJ; Yang, HL; Zhang, H; Wang, GC; Liu, K; Gu, QL; Tao, YX; Chen, GC(陈光村); Jiang, XH; Li, G; Gu, YZ; Shi, Q
通讯作者: Gu, YZ ; Shi, Q
刊名: STEM CELL RESEARCH & THERAPY
发表日期: 2016
DOI: 10.1186/s13287-016-0400-6
卷: 7
收录类别: SCI ; EI
文章类型: 期刊论文
部门归属: 纳米生物医学与安全研究部
英文摘要: Background: Mesenchymal stem cells (MSCs) are widely used in cell-based therapy owing to their multilineage potential and low immunogenicity. However, low differentiation efficiency and unpredictable immunogenicity of allogeneic MSCs in vivo limit their success in therapeutic treatment. Herein, we evaluated the differentiation potential and immunogenicity of human placenta-derived MSCs manipulated with osteogenic priming and dedifferentiation process. Methods: MSCs from human placentas were subjected to osteogenic induction and then cultivated in osteogenic factor-free media; the obtained cell population was termed dedifferentiated mesenchymal stem cells (De-MSCs). De-MSCs were induced into osteo-, chondro-and adipo-differentiation in vitro. Cell proliferation was quantified by a Cell-Counting Kit-8 or tritiated thymidine ([H-3]-TdR) incorporation. Meanwhile, the osteogenesis of De-MSCs in vivo was assayed by real-time PCR and histological staining. The expressions of stem cell markers and costimulatory molecules on De-MSCs and lymphocytes from primed BALB/c mouse with De-MSCs were determined by flow cytometry. Results: De-MSCs exhibited some properties similar to MSCs including multiple differentiation potential and hypoimmunogenicity. Upon re-osteogenic induction, De-MSCs exhibited higher differentiation capability than MSCs both in vitro and in vivo. Of note, De-MSCs had upregulated immunogenicity in association with their osteogenesis, reflected by the alternated expressions of co-stimulatory molecules on the surface and decreased suppression on T cell activation. Functionally, De-MSC-derived osteoblasts could prime lymphocytes of peripheral blood and spleen in BALB/c mice in vivo. Conclusions: These data are of great significance for the potential application of De-MSCs as an alternative resource for regenerative medicine and tissue engineering. In order to avoid being rejected by the host during allogeneic De-MSC therapy, we suggest that immune intervention should be considered to boost the immune acceptance and integration because of the upregulated immunogenicity of De-MSCs with redifferentiation in clinical applications.
关键词[WOS]: UMBILICAL-CORD BLOOD ; STROMAL CELLS ; ARTICULAR CHONDROCYTES ; BONE REGENERATION ; IMMUNE-RESPONSES ; EPITHELIAL-CELLS ; GENE DELIVERY ; IN-VITRO ; DIFFERENTIATION ; DEDIFFERENTIATION
语种: 英语
JCR小类分区: 二区
WOS记录号: WOS:000384598600004
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.sinano.ac.cn/handle/332007/4885
Appears in Collections:纳米生物医学与安全研究部_王强斌团队_期刊论文

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Recommended Citation:
Fu, XJ,Yang, HL,Zhang, H,et al. Improved osteogenesis and upregulated immunogenicity in human placenta-derived mesenchymal stem cells primed with osteogenic induction medium[J]. STEM CELL RESEARCH & THERAPY,2016,7.
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