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题名: DNA sequence-dependent fluorescence of doxorubicin for turn-on detection of biothiols in human serum
作者: Chen, X(陈星); Jiang, GM(江桂梅); Wang, ZL(王志利); Hong, SN(洪珊妮); Zhang, YY; Guo, YH; Cheng, H; Wang, JN(王金娥); Pei, RJ(裴仁军)
通讯作者: Pei, RJ(裴仁军)
刊名: ANALYTICAL AND BIOANALYTICAL CHEMISTRY
发表日期: 2016
DOI: 10.1007/s00216-015-9168-2
卷: 408, 期:3
收录类别: SCI
文章类型: 期刊论文
部门归属: 纳米生物医学与安全研究部
英文摘要: Doxorubicin (Dox) is a DNA-targeting anthracycline antibiotic active against a wide spectrum of cancers. The interaction between Dox and double-stranded DNA (dsDNA) was used to load Dox using DNA duplexes as carriers. More importantly, the interesting DNA sequence-dependent fluorescence response of Dox could be exploited in the design of efficient Dox release systems and efficient fluorescence sensors. In this work, we demonstrated that separate introduction of G and C bases into T-rich single-stranded DNA (ssDNA) sequences afforded the best discrimination of Dox binding between dsDNA and ssDNA. For the first time, we successfully utilized this interesting DNA sequence-dependent fluorescence response of Dox as a signal transduction mechanism for the sensitive detection of biothiols in human serum. Cysteine, homocysteine, and glutathione were detected at as low as 26 nM, 37 nM, and 29 nM, respectively. The biosensors exhibited not only good selectivity, stability, and sensitivity in aqueous solutions but also a sensitive response in human serum, demonstrating their potential for diagnosis.
关键词[WOS]: QUANTUM DOTS-HG(II) SYSTEM ; LABEL-FREE METHOD ; RECOVERED FLUORESCENCE ; SILVER NANOCLUSTERS ; SENSITIVE DETECTION ; GOLD NANOPARTICLES ; TOTAL HOMOCYSTEINE ; BIOLOGICAL-FLUIDS ; TOTAL CYSTEINE ; DRUG-DELIVERY
语种: 英语
JCR小类分区: 三区
WOS记录号: WOS:000368017600004
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.sinano.ac.cn/handle/332007/4912
Appears in Collections:纳米生物医学与安全研究部_裴仁军团队_期刊论文

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Recommended Citation:
Chen, X,Jiang, GM,Wang, ZL,et al. DNA sequence-dependent fluorescence of doxorubicin for turn-on detection of biothiols in human serum[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2016,408(3).
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